Uscript. Competing interests The authors declare that they have no competing
Uscript. Competing interests The authors declare that they have no competing interests. STI-571 chemical information consent for publication All authors read the manuscript thoroughly and consent to submit the manuscript to the journal of BMC Developmental Biology. Ethics approval All fish experimental procedures were performed in accordance with the Regulations for the Administration of Affairs Concerning Experimental Animals and fish sampling were approved and authorized by the Yangtze River Fish Committee in China. Received: 21 July 2016 Accepted: 6 NovemberReferences 1. Roch GJ, Busby ER, Sherwood NM. GnRH receptors and peptides: skating backward. Gen Comp Endocrinol. 2014;209:118?4. 2. Abe H, Oka Y: Neuromodulatory Functions of Terminal Nerve-GnRH Neurons. Fish Physiology. 2006;25(06):455?03. 3. Kim NN, Shin HS, Habibi HR, Lee J, Choi CY. Expression profiles of three types of GnRH during sex-change in the protandrous cinnamon clownfish, amphiprion melanopus: effects of exogenous GnRHs. Comp Biochem Physiol Part B Biochem Mol Biol. 2012;161(2):124?3. 4. Alvarado MV, Carrillo M, Felip A. Expression of kisspeptins and their Receptors, gnrh-1/gnrhr-II-1a and gonadotropin genes in the brain of adult male and female European sea bass during different gonadal stages. Gen Comp Endocrinol. 2013;187:104?6. 5. Drori S, Ofir M, Levavi-Sivan B, Yaron Z. Spawning induction in common carp (Cyprinus carpio) using pituitary extract or GnRH superactive analogue combined with metoclopramide: analysis of hormone profile, progress of oocyte maturation and dependence on temperature. Aquaculture. 1994;119(4):393?07. 6. Marte CL, Sherwood NM, Crim LW, Harvey B. Induced spawning of maturing milkfish (Chanos chanos Forsskal) with PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/27488460 PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/29072704 gonadotropin-releasing hormone (GnRH) analogues administered in various ways. Aquaculture. 1987;60(3?):303?0. 7. Mikolajczyk T, Chyb J, Sokolowska-Mikolajczyk M, Enright WJ, Epler P, Filipiak M, Breton B. Attempts to induce an LH surge and ovulation in common carp (Cyprinus carpio L.) by differential application of a potent GnRH analogue, azagly-nafarelin, under laboratory, commercial hatchery, and natural conditions. Aquaculture. 2003;223(1?):141?7. 8. Mateos J, Ma nos E, Carrillo M, Zanuy S. Regulation of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) gene expression by gonadotropin-releasing hormone (GnRH) and sexual steroids in the Mediterranean Sea bass. Comp Biochem Physiol Part B Biochem Mol Biol. 2002;132(1):75?6. 9. Hildahl J, Taranger GL, Norberg B, Haug TM, Weltzien F-A. Differential regulation of GnRH ligand and receptor genes in the brain and pituitary of Atlantic cod exposed to different photoperiod. Gen Comp Endocrinol. 2013;180:7?4.10. Hildahl J, Sandvik GK, Edvardsen RB, Fagernes C, Norberg B, Haug TM, Weltzien F-A. Identification and gene expression analysis of three GnRH genes in female Atlantic cod during puberty provides insight into GnRH variant gene loss in fish. Gen Comp Endocrinol. 2011;172(3):458?7. 11. Jodo A, Kitahashi T, Taniyama S, Ueda H, Urano A, Ando H. Seasonal changes in expression of genes encoding five types of gonadotropinreleasing hormone receptors and responses to GnRH analog in the pituitary of masu salmon. Gen Comp Endocrinol. 2005;144(1):1?. 12. Levavi-Sivan B, Avitan A. Sequence analysis, endocrine regulation, and signal transduction of GnRH receptors in teleost fish. Gen Comp Endocrinol. 2005;142(1?):67?3. 13. Lin C-J, Wu G-C, Lee M-F, Lau E-L, Dufour S, Chang C-F. Regulation of two forms of gonadot.