Proton-pump inhibitor proton-pump.com

Description:
RANK ligand (RANKL), also called TRANCE, OPGL and ODF, is a member of the TNF family that is expressed in activated T cells, lymph nodes and in stromal cell lines. RANKL (TRANCE) interacts with its receptor RANK expressed on mature dendritic cells (DC) and mature osteoclasts, leading to the inhibition of apoptosis, probably through the upregulation of bcl-x. RANKL is, therefore, a useful tool for enhancing DC and osteoclast survival and activity. Osteoprotegerin (OPG) is a potent inhibitor of RANKL.Historical data has shown that the biological activity of RANKL supports the survival of dendritic cells and osteoclasts. Figure 1: Schematic structure of human rhsRANKL (peptide, aa. 151-316). Figure 2: Binding of rhsRANKL to Osteoprotegerin. Recombinant, human sRANKL binds to Osteoprotegerin:Fc (see Prod.175799-93-6 custom synthesis No. ALX-522-007).Method: Ligand binding assay: 96 well ELISA plates were coated O/N with 50 ng OPG-Fc per well. After a blocking step SDS-PAGE Analysis of RANKL (soluble) (human), (recombinant). Lane 1: MW Marker, Lane 2: 1µg. Figure 3: Mononuclear cells differentiate into Osteoclasts in the presence of M-CSF and RANKL, Soluble (human) (recombinant) (ALX-522-012). Method: Human CD14+ mononuclear cells isolated from adult peripheral blood were cultured in control medium (upper panel), in 25ng/ml M-CSF (middle panel), or in 25ng/ml M-CSF plus 50ng/ml RANKL (lower panel). Osteoclasts were identified as Tartrate-Resistant Acid Phosphatase (TRAP)-positive multinucleated cells. Osteoclasts were detected exclusively in presence of RANKL and in these culture conditions (M-CSF + RANKL), cells fused and generated multinucleated dark red TRAP-positive cells (arrows). Nuclei were stained with haematoxylin. Original magnification: 200x. Picture courtesy of V.Kindler and D.Suvà, University of Geneva.“””,RANKL (soluble) (human) Figure 1: Schematic structure of human rhsRANKL (peptide, aa. 151-316). Figure 2: Binding of rhsRANKL to Osteoprotegerin. Recombinant, human sRANKL binds to Osteoprotegerin:Fc (see Prod. No. ALX-522-007).Method: Ligand binding assay: 96 well ELISA plates were coated O/N with 50 ng OPG-Fc per well.2226212-40-2 web After a blocking step SDS-PAGE Analysis of RANKL (soluble) (human), (recombinant).PMID:29939614 Lane 1: MW Marker, Lane 2: 1µg. Figure 3: Mononuclear cells differentiate into Osteoclasts in the presence of M-CSF and RANKL, Soluble (human) (recombinant) (ALX-522-012). Method: Human CD14+ mononuclear cells isolated from adult peripheral blood were cultured in control medium (upper panel), in 25ng/ml M-CSF (middle panel), or in 25ng/ml M-CSF plus 50ng/ml RANKL (lower panel). Osteoclasts were identified as Tartrate-Resistant Acid Phosphatase (TRAP)-positive multinucleated cells. Osteoclasts were detected exclusively in presence of RANKL and in these culture conditions (M-CSF + RANKL), cells fused and generated multinucleated dark red TRAP-positive cells (arrows). Nuclei were stained with haematoxylin. Original magnification: 200x. Picture courtesy of V.Kindler and D.Suvà, University of Geneva.“””,RANKL (soluble) (human)

Description:
Aurora A, B, and C have emerged as key mitotic regulators of mammalian centrosome function, chromosome segregation and cytokinesis. Aurora B is one of several chromosomal passenger proteins involved in chromosome alignment at the metaphase plate and cytokinesis. Phosphorylation of a threonine residue in the Aurora activation loop (Thr232 in human Aurora B) is believed to be an essential regulatory mechanism in Aurora kinase activation. IHC Analysis: Human adrenal tissue stained with [pThr232]Aurora B, pAb at 5µg/ml.{{6090-95-5} site|{6090-95-5} Purity & Documentation|{6090-95-5} Formula|{6090-95-5} custom synthesis} IHC Analysis: Human skin tissue stained with [pThr232]Aurora B, pAb at 5µg/ml. IHC Analysis: Human adrenal tissue stained with [pThr232]Aurora B, pAb at 5µg/ml. IHC Analysis: Human skin tissue stained with [pThr232]Aurora B, pAb at 5µg/ml.

InChiKey:
MIERMBQDBLFAFW-UHFFFAOYSA-N

Description:
Phosphatidylinositol 3-kinase (PI3-K) is a lipid kinase that phosphorylates the inositol ring of phosphatidylinositol and related compounds at the 3 Western blot analysis of PI3 kinase expression in Jurkat (1), Raji (2), U937 (3), WR19L (4) and PC12 (5) using PI3-kinase, p85a mAb (AB6). Western blot analysis of PI3 kinase expression in Jurkat (1), Raji (2), U937 (3), WR19L (4) and PC12 (5) using PI3-kinase, p85a mAb (AB6).

Description:

InChiKey:
XVPBINOPNYFXID-ACTGZQEFSA-N

Description:
Distinguishes between live vs dead cells in a single reagent. The NUCLEAR-ID® Blue/Green cell viability reagent is a mixture of a blue fluorescent cell-permeable nucleic acid dye and a green fluorescent cell-impermeable nucleic acid dye that is suited for staining dead nuclei. The staining pattern arising from the simultaneous combination of these two dyes permits determination of live and dead cell populations by fluorescence/confocal microscopy. The reagent, supplied as a 1000x solution, is sufficient for 1000 microscopy assays. The single-tube format makes this cell viability reagent easy to use. It leaves the cytoplasm unstained, potentially allowing visualization of other cell markers.{{1801765-04-7} MedChemExpress|{1801765-04-7} Purity & Documentation|{1801765-04-7} Formula|{1801765-04-7} manufacturer} Figure 1: The NUCLEAR-ID® Blue/Green dye is detected as blue-stained nuclei in live cells and fluorescent-green nuclei in dead cells (arrows).{{1124329-14-1} web|{1124329-14-1} Protocol|{1124329-14-1} In Vitro|{1124329-14-1} manufacturer} Figure 1: The NUCLEAR-ID® Blue/Green dye is detected as blue-stained nuclei in live cells and fluorescent-green nuclei in dead cells (arrows).PMID:25905396

Description:

InChiKey:
BVUJISIVAHYNLI-FIBGUPNXSA-N

Description:
Dihydrofolate reductase inhibitor Folic acid antagonist. Potent inhibitor of dihydrofolate reductase.{{1393477-72-9} site|{1393477-72-9} Protocol|{1393477-72-9} References|{1393477-72-9} supplier} Induces apoptosis. Inhibits DNA synthesis. Anticancer agent.