Enhances the release of endothelial progenitor cells in nearby chest-irradiated mice Hargita Hegyesi1; Nikolett S dor2; Violetta L er3; G a S r y3; Vir Lovas1; Tam Visnovitz1; CXCR2 Antagonist list Krisztina P zi4; Lilla Turiak5; L d Bert three; Edit I. BuzSemmelweis University Department of Genetics, Cell- and Immunobiology, Budapest, Hungary; 2National Public Wellness Center National Analysis Directorate for Radiobiology and Radiohygiene, Budapest, Hungary; 3 National Public Wellness Center National Investigation Directorate for Radiobiology and Radiohygiene, Anna st 5, Hungary; 4Department ofISEV 2018 abstract book Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary; 5Research Centre for Organic Sciences, Hungarian Academy of Sciences, Budapest, Hungary; 6MTA-SE Immune-Proteogenomics Extracellular Vesicle Study Group, Budapest, HungaryLBT03.07 = OWP2.Immunofluorescence flow cytometry of extracellular vesicle surface proteins John Nolan; Erika Duggan Scintillon Institute, San Diego, CA, USABackground: Because the incidence of breast cancer continues to rise, the use of radiotherapy (RT) has emerged as a major therapy modality. Even so, RT also increases the risk of coronary heart illness and cardiac mortality. Various research have demonstrated the protective effects of radio-detoxified endotoxin (RD-LPS) in lowering chemotherapy- and radiation-BRD2 Inhibitor Gene ID induced cardiac damages. Bone-marrow (BM) derived endothelial progenitor cells (EPCs) have been shown to have regenerative possible in endothelial injuries. In our chest-irradiated mouse model here we investigated if exosomes (EXOs) could play a function in RD-LPS induced EPC activation. Solutions: Hearts of C57BL/6 mice received a 16 Gy single dose of X-ray radiation. In this mouse model of RT-induced cardiac injury, we quantified RD-LPS treated BM derived EXOs, analysed their proteomic composition by MS, measured IFITM3 protein levels in BM derived EXOs released following RD-LPS remedy by an ELISA. EPCs (CD31+ or FLK-1+) and CD34+ hematopoietic stem cells (HCS) were immunophenotyped both in blood and BM samples by flow cytometry. Outcomes: Mice showed elevated lethality right after 16 Gy nearby chest irradiation, when RD-LPS treatment prolonged their median survival drastically. Each in BM and circulation from the exposed and RD-LPS treated groups, the number of EPCs and HCS have been higher than within the nonirradiated mice. MS final results demonstrated that BM EXO proteins in RDLPS treated mice integrated both a widespread set of EXO proteins and precise subsets of treatment-related proteins which include interferon-induced transmembrane protein-3 (IFITM3), which correlated with treatmentassociated functions. Flow cytometry and ELISA assessment of EXOs secreted by BM cells of RD-LPS treated mice, revealed a distinction inside the expression of IFITM3 involving EXOs released within the presence or absence of RD-LPS. Summary/Conclusion: That is the very first study to demonstrate that RDLPS remedy induces migration of EPCs into the circulation, which results in an attenuated RT mortality. EPC activation is dependent on RD-LPS treatment, which leads to IFITM3 upregulation within the BM derived EXOs. Our information suggest that EXO IFITM3 may well play a function and serve as a prospective biomarker in cardiac regeneration. Funding: This operate was supported by National Investigation, Improvement and Innovation Fund of Hungary; using the following grants [NVKP_161-2016-0017].Background: Just like the cells that generate them, extracellular vesicles (EVs) bear surface molecules that ca.