Ith the comparedntioxidants 2021, ten, x FOR PEER REVIEWFigure 3. Liver GNF6702 Data Sheet indices of indices
Ith the comparedntioxidants 2021, ten, x FOR PEER REVIEWFigure three. Liver indices of indices of channel catfish (Ictalurus punctatus) topic to distinctive treatFigure three. Liver antioxidant antioxidant channel catfish (Ictalurus punctatus) topic to various remedy. (A) Malonment.(B) Superoxide dismutase, SOD; (C) Glutathione peroxidase, GPx; (D) Glutathione reductase, GR; dialdehyde, MDA; (A) Malondialdehyde, MDA; (B) Superoxide dismutase, SOD; (C) Glutathione peroxidase, GPx; (D) Glutathione reductase, GR; (E) Glutathione, GSH; (F) Total antioxidant capacity, T-AOC. (E) Glutathione, GSH; (F) Total antioxidant capacity, T-AOC. Data indicate the mean values of three replicate cages per Information indicate the mean values of three replicate cages per remedy (three fish per replicate breedtreatment (three fish per replicate breeding barrel). SignificanceANOVA (p 0.05) followed by Duncan’s multi- followed by ing barrel). Significance was evaluated by one-way was evaluated by one-way ANOVA (p 0.05) Duncan’s various variety tests. Values marked with unique letters are considerably distinctive among the treatple variety tests. Values marked with distinctive letters are substantially distinctive amongst the remedy groups. ment groups.3.six. Antioxidant-Related Gene Expression inside the Liver As shown in Figure four, there was no significant difference in sod and gpx8 gene expression amongst the remedy groups. The OFO group remarkably LY294002 Biological Activity downregulated gpx1, gr, and nrf2 mRNA expression, and upregulated keap1 mRNA expression (p 0.05) com-Antioxidants 2021, 10,9 of3.six. Antioxidant-Related Gene Expression within the Liver As shown in Figure four, there was no important difference in sod and gpx8 gene expression among the therapy groups. The OFO group remarkably downregulated gpx1, gr, and nrf2 mRNA expression, and upregulated keap1 mRNA expression (p 0.05) compared with all the FFO group. gpx1, gr, and nrf2 mRNA expression inside the FFOT group have been considerably Antioxidants 2021, ten, x FOR PEER Critique 10 of 20 upregulated compared with all the OFO group, whereas the opposite outcome was observed for the mRNA expression degree of keap1 (p 0.05)Figure 4. Effects of dietary taurine on liver antioxidant-related genes expression of channel catfish (Ictalurus punctatus) fed Figure 4. Effects of dietary taurine on liver antioxidant-related genes expression of channel catfish (Ictalurus punctatus) fed oxidized-fish-oil diets. (A) sod; (B) gpx1; (C) gpx8; (D) gr; (E) keap1; (F) nrf2. Data indicate the mean values of three replicate oxidized-fish-oil diets. (A) sod; (B) gpx1; (C) gpx8; (D) gr; (E) keap1; (F) nrf2. Data indicate the imply values of three replicate cages per therapy (3 fish per replicate breeding barrel). Significance was evaluated by one-way ANOVA (p 0.05) cages per remedy (three fish per replicate breeding barrel). Significance was evaluated by one-way ANOVA (p 0.05) followed by Duncan’s numerous range tests. Values marked with different letters are substantially distinctive amongst the followed by Duncan’s several range tests. Values marked with different letters are substantially different between the therapy groups. therapy groups.3.7. Histological Structure in the Intestine 3.7. Histologicalin Figure five, via the H E staining evaluation with the intestine, the feeding As shown Structure in the IntestineAs shown in Figure drastically H E staining analysis of your intestine, the feeding of of oxidized-fish-oil diets5, through the decreased the goblet.