Was distinctive between[27and SPS. Miller MS content material, pH, organic, and
Was distinct between[27and SPS. Miller MS content material, pH, organic, and inorganic nutrient contents influence AMF spore germination (2000) this experiment,thatsoil moisture content was various involving MS and SPS. Mil- et al., (2009) [32] [31] showed the FM4-64 site flooding partially inhibits AMF colonization. Wang 30]. In found a [31] showed that floodingbetween AMFAMF colonization. Wang et al. (2009) ler (2000) damaging correlation partially inhibits colonization and moisture content material, since AMF [32] discovered a damaging correlation between these prior findings, SPS’s higher need to have oxygen to thrive. ConsideringAMF colonization and moisture content material, given that moisture content material AMF need oxygen could possibly be 1 of to thrive. Considering these prior findings, SPS’s higher moisture the aspects limiting the AMF colonization rate.content may possibly be on the list of elements limiting the AMF colonization rate.(cm) 300 05 4.six 05 4.1 150 Abundance of4.0 AMF 300 four.15pH (H2O)38.1 1.03 79.8 0.20 77.7 Colonization 0.23 50.six 0.1.03 66.2 16.9 C N P2O5 0.20 69.7 2.7 Content material Content material (Bray II) 0.23 m-3) 64.9 (g m-3) 1.four (kg 0.96 52.7 18.66.2 69.7 64.9 52.7 16.9 2.7 1.4 18.7 1.6 1.9 3.two 4.Agriculture 2021, 11, x FOR PEER REVIEWFigure 1. Photomicrograph SPS,root samples stained with acid fuchsin.2:The best photographs plus the tom photos were roots in MS and of respectively. Bar = 100 ; arrow 1: hypha; arrow vesicle; five of 9 arrow 3: pictures had been spore. bottom arbuscule; arrow four:roots in MS and SPS, respectively. Bar = 100 ; arrow 1: hypha; arrow 2: vesicle; arrow 3: arbuscule; arrow 4: spore.Figure 1. Photomicrograph of root samples stained with acid fuchsin. The top photos as well as the bot-AMF colonization rate MSSPSFigure 2. AMF colonization prices inside the roots the roots of sago palm grown in indicate Figure 2. AMF colonization prices in of sago palm grown in MS and SPS. Bars MS and SPS. Bars indicate typical deviation in the signifies (n = 5). indicate levels in the 0.001 probability level according to normal deviation from the means (n = 5). indicate levels at the 0.001 probability level based on the Student t-test.the Student t-test.three.3. AMF Neighborhood Structure A total of 122 AMF operational taxonomic units (OTUs) containing 78 OTUs in the MS sample and 50 OTUs in the SPS sample (six Icosabutate supplier shared OTUs) have been obtained. Those OTUs were incorporated in to the neighbor-joining tree with AMF reference sequences in the NCBI-GenBank, and 17 clades have been formed (Figure 3). The 122 AMF sequencesAgriculture 2021, 11,five of3.three. AMF Neighborhood Structure A total of 122 AMF operational taxonomic units (OTUs) containing 78 OTUs from the MS sample and 50 OTUs in the SPS sample (six shared OTUs) have been obtained. Those OTUs have been incorporated in to the neighbor-joining tree with AMF reference sequences from the NCBI-GenBank, and 17 clades had been formed (Figure 3). The 122 AMF sequences had been classified into Acaulosporaceae, Ambisporaceae, Claroideoglomeraceae, Gigasporaceae, and Glomeraceae; Glomus was dominant in MS (76 ), and Acaulospora and Glomus were dominant in SPS (37 and 63 , respectively) (Table 2). Clades Aca 2, Aca three, Cla, Glo 2, Glo 3, Glo 5, Glo 6, and Glo 8 only have OTUs from the MS sample, when clades Aca 4 and Gig six of 9 only have OTUs in the SPS sample. In addition, the number of belonging clades was larger in MS (15) than in SPS (7).OTU046, 126, 081 (MS), OTU004,071,089 (SPS) OTU088 SPS Uncultured Glomus (LT216791) OTU053 SPS OTU005 SPS OTU013 SPS OTU003, 019, 062, 102 (SPS) OTU064 (MS), OTU.