NUCLEAR-ID® Blue/Green cell viability reagent
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NUCLEAR-ID® Blue/Green cell viability reagent
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≥93% (HPLC)
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Distinguishes between live vs dead cells in a single reagent. The NUCLEAR-ID® Blue/Green cell viability reagent is a mixture of a blue fluorescent cell-permeable nucleic acid dye and a green fluorescent cell-impermeable nucleic acid dye that is suited for staining dead nuclei. The staining pattern arising from the simultaneous combination of these two dyes permits determination of live and dead cell populations by fluorescence/confocal microscopy. The reagent, supplied as a 1000x solution, is sufficient for 1000 microscopy assays. The single-tube format makes this cell viability reagent easy to use. It leaves the cytoplasm unstained, potentially allowing visualization of other cell markers.{{1801765-04-7} MedChemExpress|{1801765-04-7} Purity & Documentation|{1801765-04-7} Formula|{1801765-04-7} manufacturer} Figure 1: The NUCLEAR-ID® Blue/Green dye is detected as blue-stained nuclei in live cells and fluorescent-green nuclei in dead cells (arrows).{{1124329-14-1} web|{1124329-14-1} Protocol|{1124329-14-1} In Vitro|{1124329-14-1} manufacturer} Figure 1: The NUCLEAR-ID® Blue/Green dye is detected as blue-stained nuclei in live cells and fluorescent-green nuclei in dead cells (arrows).PMID:25905396
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| Application Flow Cytometry, Fluorescence microscopy, Fluorescent detection | Purity ≥93% (HPLC) | Quantity 100μl (for 1000 microscopy assays). | Technical Info / Product Notes Wavelength MaximaLive (Blue): Excitation: 350nm; Emission: 461nmDead (Green): Excitation: 503nm; Emission: 524nmThe NUCLEAR-ID® Blue/Green cell viability reagent is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.