Stained with Staining solutionHuman Molecular Genetics, 2014, Vol. 23, No.(concentrated Rinse buffer containing 5 mM potassium ferricyanide, 5 mM potassium ferrocyanide and 1 mg/ml X-gal) at 378C for 48 h. The stained slices were then rinsed in PBS supplemented with 2 mM MgCl2 and mounted onto glass slides working with Vectashield (Vector Laboratories). The sections have been imaged applying an Axiovert microscope (Zeiss) equipped with all the PDE11 Synonyms AxioVision application. The photos of the different portions from the cerebellum were captured employing a ALK3 Compound 4objective and merged collectively working with the ImageJ software program to obtain a composite picture in the whole structure.SUPPLEMENTARY MATERIALSupplementary Material is available at HMG on the internet.ACKNOWLEDGEMENTSWe thank members from the Opal lab for their intellectual input. P.O. thanks Dr Ameet Kini for discussions and important reading from the manuscript. We thank Jessica Huang for enable with histopathology and mouse genotyping. We also thank the Northwestern University Behavioral Phenotyping Core for enable with behavioral assays, and also the Northwestern University Mouse Histology and Phenotyping Laboratory for aid with staining. We thank Dr Kwang-Youn Kim within the Biostatistics Core for advice on statistical tests. Conflict of Interest statement. None declared.FUNDINGThis perform was funded by the US National Institutes of Health (grant nos R01 NS062051 and 1R01NS082351); with added funding in the National Ataxia Foundation as well as the Brain Investigation Foundation (P.O.).