Ibility criteria for this study received informations concerning the protocols. Each participant who met the criteria answered the 5 questions, receiving enable from nurses or physicians. All participants underwent basal spirometry (Sensor Medics, Yorba Linda, CA. USA). The following parameters were measured: FEV1, FVC, and FEV1/FVC. The highest FEV1 was chosen amongst three consecutive procedures with basal spirometry. Subjects using a basal FEV1 of far more than 70 in the predictive value by spirometry underwent MBPTs. Before the MBPT, subjects were asked to discontinue any medications that could interfere together with the methacholine test. The diagnosis of asthma was confirmed according to a constructive response towards the MBPT (PC20 16 mg/dL of inhaled methacholine). The incremental concentrations of methacholine chloride prepared in the dosing protocol have been 0.0625, 0.25, 1, four, 16, 25, and 50 mg/mL. A lower of 20 in the baselineLim et al. BMC Pulmonary Medicine 2014, 14:161 http://www.biomedcentral/1471-2466/14/Page three ofFEV1 using a dose of 16 mg/mL of methacholine was regarded a positive response. Methacholine was inhaled applying the 2-min tidal breathing strategy using a synchronized nebulizer or five-breath dosimeter process (DSM-2) in line with ATS recommendations. Spirometry was repeated 3 min after each and every increased dose of methacholine. After the methacholine test, all participants received salbutamol and repeated spirometry was performed to assess recovery of lung function. Individuals had been divided into two groups, asthmatics and non-asthmatics, in accordance with the outcomes in the MBPT. Patients have been diagnosed with asthma if their answers towards the questionnaire recommended it along with the MBPT was constructive. The relationship among asthma symptoms and also the presence of BHR was determined by the sensitivity (proportion of sufferers with BHR who had a good questionnaire result) and specificity (proportion of sufferers with normal responsiveness who had a unfavorable questionnaire outcome). The baseline traits from the asthmatics and non-asthmatics are shown in Table 1. This study protocol was authorized by the Institutional Overview Board (Approval No. ECT198-2-16) of Ewha Womans University Mokdong Hospital and we received written informed consent from participants.Asthma screening five-item questionnaire according to GINAStatistical analysisThe imply total symptom scores for the two groups have been compared employing Student’s t-test. Multivariate logistic regression analysis was performed to identify whether or not the 5 concerns employed as independent variables could substantially differentiate asthmatics and non-asthmatics.Raltitrexed The correlation in between the questionnaire and asthma was defined by the odds ratios (OR) and 95 confidence intervals (CI).MK-6240 A receiver-operating characteristic (ROC) curve analysis was performed to assess the diagnostic accuracy with the symptom-assisted diagnosis.PMID:23664186 A p worth significantly less than 0.05 was thought of to indicate statistical significance. Statistical analyses have been performed working with SPSS version 16.0 (SPSS, INC, Chicago, IL, USA).Q1. Has the patient had an attack of wheezing Q2. Does the patient have wheeze or dyspnea following exercising Q3. Does the patient possess a troublesome cough at night Q4. Did the patient’s cold take much more than ten days to clear up Q5. Did the patient practical experience wheezing, chest tightness, or cough immediately after exposure to airborne allergens or pollutantsTable 1 Baseline traits of subjects who underwent MBPT and completed questionnaireCharacteristic Me.