Tients with diabetes. Approaches: Sufferers at Concord Hospital with suspected CAD gave written informed consent and had been administered RIPC (sphygmomanometer around the arm, 3 5 min cycles, n = 31) or sham (n = 29) prior to angiography, with recruitment ongoing. Blood was collected pre- and immediately post-RIPC/sham and plateletfree plasma generated. International coagulation/fibrinolytic potential was measured by NMDA Receptor Molecular Weight general haemostatic possible assay (Reddel et al. Thromb Res. 2013; 131(5): 457462) and a variety of fibrinolytic things by ELISA. EV wereUniversity College Dublin, Dublin, Ireland; bQueen Mary University of London, London, UK; cThe Mater Misericordiae University Hospital, Dublin, Ireland; dWilliam Harvey Study institute, Queen Mary University of London, London, UKIntroduction: Urinary extracellular vesicles (uEVs) (exosomes, microvesicles and apoptotic bodies) have possible as diagnostic and prognostic biomarkers. In atherosclerosis, the underlying result in of heart attack and stroke, EV release might be dysregulated and their contents can mediate pro-inflammatory effects. Several markers have already been previously identified on uEV which includes exosome markers CD63 and CD9, CD45 (leukocyte marker), CD61 (platelet marker), CD14 (monocyte/macrophage marker) and / integrins. The selectively packaged cargo of those membrane bound carriers consist of microRNAs (miRs). miR-21 and miR-155 are important regulatory miRs which might be upregulated in immune cells and are released in EVs following exposure to pro-inflammatory stimuli. miR-155 has been reported to possess pro-atherogenic effects and miR-155 deficiency in murine models leads to reduced atherosclerotic lesion burden.ISEV2019 ABSTRACT BOOKMethods: Urine was collected from patients diagnosed with coronary artery disease (CAD), classified as symptomatic (non-ST-elevation myocardial infarction, STelevation myocardial infarction or unstable angina) or asymptomatic (stable angina). uEVs from symptomatic and asymptomatic patients have been isolated via benchtop centrifugation. The concentration and size of uEVs were analysed through the NanoSight NS300 (n = 15 per group). The expression of miR-155 and miR-21 was investigated by RT-qPCR (n = 10 per group). uEV surface marker expression was analysed by ImageStreamX MK2 Imaging Flow Cytometer (12 per group). Results: uEV concentration in symptomatic individuals (median; 6.46E+9 particles/mL) was significantly decreased (p 0.05) compared to asymptomatic individuals (median; 1.25E+10 particles/mL). CD11B+ uEVs were improved and CD16+ uEVs had been decreased in the symptomatic patients (p 0.01). Furthermore, the concentration of CD45+ EVs were increased in symptomatic individuals (p 0.001). Though uEV miR-21 was unchanged, miR-155 expression was significantly increased inside the symptomatic group (p 0.05). Summary/Conclusion: uEV concentration, miR-155 expression and surface marker expression have diagnostic and prognostic prospective. As CAD severity increases, uEV concentration is decreased, surface marker expression is altered and uEV miR-155 expression is improved. Funding: The Irish Investigation Council.OT01.Circulating extracellular vesicle-associated microRNAs as predictive biomarkers of cardiovascular complications in end-stage renal disease Dakota D. Gustafsona, Jessica Fitzpatrickb, Jason Fishc and Rulan Parekhba PRMT8 manufacturer Division of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada; bChild Well being Evaluative Sciences, Investigation Institute, The Hospital for Sick Children,.