Twork. The dendrogram indicated that the Ensitrelvir SARS-CoV yellow module had the highest association with all the nematode population (Figure 4c).Figure four. Module eigengene expression pattern and correlation. (a) Comparison of eigengene expression (y-axis) in the turquoise module across the samples (x-axis); (b) comparison of eigengene expression in the yellow module across the samples; (c) hierarchical clustering dendrogram and heatmap of the correlated eigengenes and nematode population. ME, module eigengene.two.four. Functional and Pathway Enrichment Analyses of Genes inside a Nematode Population-Related Module GO enrichment and KEGG enrichment analyses had been performed around the genes in the turquoise and yellow modules to identify the functions of these genes plus the pathways that may well be involved. GO enrichment analysis revealed that genes within the turquoise and yellow modules had been primarily enriched in catalytic activity (GO:0003824) and binding (GO:0005488) within the molecular function category; in cell (GO:0005623), cell aspect (GO:0044464), and intracellular (GO:0005622) within the cellular component category; and metabolic approach (GO:0008152) inside the biological method category. Also, a lot of genes within the turquoise module have been enriched in cellular nitrogen compound metabolic course of action (GO:0034641), gene expression (GO:0010467), localization (GO:0051179), heterocycle metabolic approach (GO:0046483), adenyl ribonucleotide binding (GO:0032559), and nucleic acid binding (GO:0003676, Table S3). Even so, several genes in the yellow module had been enriched inInt. J. Mol. Sci. 2021, 22,7 ofoxidoreductase activity (GO:0016491), transition metal ion binding (GO:0046914), organic acid metabolic process (GO:0006082), biosynthetic procedure (GO:Latrunculin A Cancer 0009058), carboxylic acid metabolic method (GO:0019752), and oxoacid metabolic procedure (GO:0043436, Table S4). Depending on the KEGG enrichment analysis, genes within the turquoise and yellow modules have been primarily enriched in metabolic pathways (ko01100) and biosynthesis of secondary metabolites (ko01110). Furthermore, several genes inside the turquoise module have been enriched in the ribosome (ko03010), spliceosome (ko03040), plant hormone signal transduction (ko04075), protein processing in endoplasmic reticulum (ko04141), carbon metabolism (ko01200), plant athogen interaction (ko04626), RNA transport (ko03013), and MAPK signaling pathway plant (ko04016, Table S5). Even so, many genes in the yellow module were enriched in flavonoid biosynthesis (ko00941), biosynthesis of amino acids (ko01230), and phenylpropanoid biosynthesis (ko00940, Table S6). 2.5. Acquiring Genes with High Gene Significance and High Intramodular Connectivity in Fascinating Modules To quantify the similarity of genes within the turquoise module as well as the yellow module, the associations of person genes with nematode populations were quantified by defining gene significance (GS) because the correlation between the gene and also the trait, along with a quantitative measure of module membership (MM) was also defined as the correlation with the ME plus the gene expression profile for the module. A scatterplot of GS vs. MM inside the turquoise module plus the yellow module was plotted, and GS and MM have been very correlated, illustrating that genes very substantially associated having a trait had been generally also by far the most vital (central) components of modules associated with all the trait (Figure 5a,d).Figure 5. Relationship amongst gene significance (GS), module membership (MM), and intramodular connectivity (IC). (a) GS for the nematode popul.