S of TNF IL10, and on the suppressor of cytokine signalling SOCS-3 had been detected and quantified by competitive RT-PCR and by Western blot. DNA binding activity on the transcription order KPT-8602 (Z-isomer) components NF-B and STAT-3 was detected by eletrophoretic-mobility-shift assay (EMSA) and super shift, and phosphorylation of IB- and STAT-3 by Western blot. CellularP148 Methylprednisolone sodium succinate reduces postoperative hyperthermia but does not affect cardiac function soon after aortic valve replacementR Jara-Rubio*, J Galcer?, E Serrano, J Diaz, LF Carbonell *Intensive Care Unit, Hospital Universitario Arrixaca, 30120 Murcia, Spain; Departamento de Fisiolog , Universidad de Murcia, Spain Objective: Steroids happen to be applied in cardiac surgery for many years to cut down the inflammatory response related with extracorporeal circulation. On the other hand, their clinical advantages have not been properly established. The aim of the present study was to evaluate the impact of methylprednisolone on body temperature and cardiac index in the postoperative period of aortic valve replacement with extracorporeal circulation and aortic clamp. Individuals: Twenty-two consecutive patients undergoing aortic valve replacement and extracorporeal circulation. The individuals received methylprednisolone (30 mg/kg; n = 11, MP group) or received no medication (n = 11, no MP group) before cardiopulmonary bypass. Clinical and demographic qualities of the sufferers were related in both groups.Accessible on the internet http://ccforum.com/supplements/6/SFigureMP37.09 37.FigureNo MP37.MPNo MPCI (l/min/m2)36.63 35.T36 3535.4 Hours4 Hours4 Hours4 HoursMeasurements and key results: Physique temperature (To, oC) and cardiac index (CI, l/min/m2) had been measured within the postoperative period on admission in the ICU and at four hours and 8 hours following ICU admission. On admission to ICU there had been no variations in temperature in between groups. Body temperature was significantly decrease in group MP at 4, and eight hours after ICU admission (P < 0.05) (Fig. 1).Cardiac index increased during the postopera-tive period, with no significant differences between groups (Fig. 2). Conclusion: Methylprednisolone sodium succinate reduces hyperthermia without affecting cardiac function in the postoperative period of aortic valve replacement with extracorporeal circulation and aortic clamp.P149 Hemostatic and fibrinolysis markers in serum and shed mediastinal blood after elective coronary artery bypass graftingAA Peivandi*, M Dahm*, D Peetz, W Kasper-Koenig*, H Oelert* *Department of Cardiothoracic and Vascular Surgery, and Institute of Clinical Chemistry, University Hospital Mainz, D-55131 Mainz, Germany Objective: Reduction of homologous blood products in cardiac surgery is mainly achieved by autologous blood salvage. One of the most customary methods consists in autotransfusion of shed mediastinal blood within the first 6 hours after surgery. Aim of this prospective study was to compare serum and shed mediastinal blood qualities of hemostatic and fibrinolysis markers early after elective coronary artery bypass grafting (CABG). Methods: Forty-seven patients (mean age 68.1 ?6.9, 15 female/ 32 male) underwent first-time elective CABG with extracorporal circulation via median sternotomy. Activated partial thromboplastin time (aPTT), prothrombin time (Quick's value), international normalized ratio (INR), thrombin time, and fibrinogen (factor I) in arterialTable PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/20724694 1 Right after admission for the ICU Shed blood Quick’s value ( ) INR APTT (s) Thrombin time (s).